1983 Volume 93 Issue 3 Pages 833-838
In order to clarify the enzymatic properties of human salivary kallikrein, we investigated its actions on human plasma kininogens and response to proteinase inhibitors. Salivary kallikrein released kinin from both LMW and HMW kininogens, but acted preferentially on LMW kininogen. Salivary kallikrein did not completely release the kinin from HMW kininogen even after prolonged incubation (up to 60 min). The kinin releasing activity of salivary kallikrein was not inhibited by plasma proteinase inhibitors such as α2M, α2PI, AT III, and C1 INA after preincubation for 60min at 37°C, but was inhibited progressively by α1AT as a function of prein-cubation time. SDS-PAGE revealed that concomitantly with the progressive inhibition a new band of 92, 000 daltons, which seemed to be a complex of salivary kallikrein and α1AT, was produced. The kininogen-depleted plasma inhibited the salivary kallikrein to the same degree as α1AT. Therefore, it is suggested that α1AT is the only inhibitor of salivary kallikrein in human plasma.
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