1983 Volume 93 Issue 5 Pages 1457-1460
A mouse hybrid cell line producing an antibody to human nervous system-specific γγ enolase has been isolated by fusions between γγ-immunized mouse spleen cells and mouse myeloma cells (P3-NS-1/1-Ag4-1), followed by a screening procedure with an enzyme immunoassay. This particular cell line (El-G3) has secreted the antibody bearing γ2a/κ immunoglobulin chains. Specificity of the E1-G3 antibody was tested by immunoprecipitation of enolase activities with anti-mouse IgG, and by use of enzyme immunoassay systems for enolase isozymes which consisted of polyclonal rabbit antibodies. The E1-G3 antibody was found to be specific for the γ subunit of enolase, showing reactivities with human γγ and αγ enolases, and also with rat γγ enolase. However, the monoclonal antibody did not cross-react with the α or β subunit of human enolase.
