Identification of Subunits of RNA Polymerase II from Ehrlich Ascites Tumor Cells

Abstract

A procedure was developed for large scale purification of RNA polymerase II from Ehrlich ascites tumor cells. About 2mg of purified enzyme was obtained from 800g of wet cells. Ten subunits were identified which behaved corresponding to the enzyme activity both on DEAE-Sephadex chromatography and on glycerol density gradient centrifugation. Analysis of tryptic peptides of each subunit showed that these subunits were independent proteins and not structurally related to each other.