A New Packing for Separation of DNA Restriction Fragments by High Performance Liquid Chromatography

Yoshio KATO; Masaaki SASAKI; Tsutomu HASHIMOTO; Tomoaki MUROTSU; Shinichi FUKUSHIGE; Kenichi MATSUBARA

Yoshio KATO, Masaaki SASAKI, Tsutomu HASHIMOTO, Tomoaki MUROTSU, Shinichi FUKUSHIGE, Kenichi MATSUBARA

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JOURNAL FREE ACCESS

1984 Volume 95 Issue 1 Pages 83-86

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Abstract

TSK-GEL SW was found to be useful as a packing in high performance liquid chromatography for the separation of double-stranded DNA restriction fragments. DNA fragments smaller than 300 base pairs were separated as discrete peaks depending solely upon difference in chain length. The recovery of DNA fragments was higher than 90%.

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