1984 Volume 95 Issue 3 Pages 611-617
A simple method has been developed for the large scale purification of neuronspecific enolase [EC 4. 2. 1. 11]. The method consists of ammonium sulfate fractionation of brain extract, and two subsequent column chromatography steps on DEAE Sephadex A-50. The chromatography was performed on a short (25cm height) and thick (8.5cm inside diameter) column unit that was specially devised for the large scale preparation. The purified enolase was crystallized in 0.05M imidazole-HCl buffer containing 1.6M ammonium sulfate (pH 6.39), with a yield of 0.9g/kg of bovine brain tissue.
