Characterization of N-Glycolylneuraminic Acid-Containing Glycosphingolipids from a Marek's Disease Lymphoma-Derived Chicken Cell Line, MSB 1, as Tumor-Associated Heterophile Hanganutziu-Deicher Antigens

Abstract

Heterophile, Hanganutziu-Deicher (HD) antigen-active N-glycolylneuraminic acidcontaining glycosphingolipids (GSLs) were detected as tumor-associated foreign antigens of a Marek's disease lymphoma-derived cell line, MSB 1, by enzyme-immunoassay with chicken antibody against N-glycolylneuraminyl-lactosylceramide (anti-NeuGc-LacCer). At least three species of HD antigen-active GSLs were detected by two-dimensional thin-layer chromatography (TLC) combined with enzyme-immunoassay. The reactivities of the GSLs with anti-NeuGc-LacCer, their behaviors on two-dimensional TLC and the results of an endo-β-galactosidase digestion study indicated that these three GSLs were NeuGc-LacCer (NeuGcα2-3Galβ1-4Glc-Cer), NeuGc-nLcOse₄Cer (NeuGcα2-3Galβ1-4GlcNAcβ1-3Galβ1-4Glc-Cer) and NeuGc-nLcOse₆Cer (NeuGcα2-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glc-Cer).