Nucleotide Sequence Involved in the Replication of Cloned Yeast Mitochondrial DNA
1984 Volume 96 Issue 1 Pages 171-177
Details
1984 Volume 96 Issue 1 Pages 171-177
A fragment of yeast mitochondrial DNA, Alu B, has two subfragments, Alu B 1 and Alu B 2. They were each cloned and sequenced. The autonomously replicating function of the curtailed Alu B 1 (342 bp) was defined within 186 bp. A GC-rich sequence identical to the oris sequence in the curtailed Alu B 1 was unnecessary for its autonomously replicating function. The 186 bp sequence had an ATATAAAT sequence and the stem and loop structures.
The base sequence of Alu B 2 also contained the same octanucleotides, the stem and loop structures, one oris sequence and one unique GC cluster. Yeast transformants with cloned Alu B 2 grew slowly. The cloned Alu B 2 was enlarged in the yeast host concomitantly with compensation of the slow growth of the transformants.
