Molecular Cloning and Nucleotide Sequence of DNA Complementary to Rat Ribosomal Protein S 26 Messenger RNA

Abstract

A cDNA clone specific for rat ribosomal protein S 26 was isolated by a positive hybridization translation assay from a cDNA library made for 8-9 S poly(A)mRNA from regenerating rat liver. The nucleotide sequence of the cDNA was determined. The sequence contains 23 base pairs in the 5' noncoding region, 345 base pairs in the protein coding region and 67 base pairs in the 3' noncoding region besides the poly(A) tail. The primary structure of the protein S 26 was deduced from the nucleotide sequence. It consists of 115 amino acids. Its molecular weight is 13, 015 and its pI is about 11.1. The calculated amino acid composition is consistent with the reported composition of S 26. From the results of Southern blot analysis, the protein S 26 appears to have multiple genes.