Abstract
This study evaluated the effects of different implant surface roughness and implant materials on bacterial
attachment and biofilm formation.
The 4 different surface-treated titanium plates (17.0 mm × 10.0 mm × 1.0 mm) were used including MS:
Machined surface, SBA: Sandblasted-acid etched surface, RA1.5: HA sputter-coated surface and RA3.0: HA
sputter-coated surface (rougher surface than RA1.5). Thirty samples were fabricated for experiments on
each of the different surfaces. Two bacterial species were used in this study: Streptococcus mutans and
Porphyromonas gingivalis. S. mutans was cultured in BHI agar or broth mediums containing 1% of sucrose. P.
gingivalis was cultured in horse blood agar mediums containing hemin and menadione and BHI broth coutaing
them under an anaerobic condition at 37°C.
Evaluation Method: The mean value of the colony forming unit (CFU) attached to each sample was
calculated from data obtained and compared to that of different implant materials. Regarding S. mutans, there
was no difference between SBA and MS. However, when compared with SBA, the values of CFU of RA1.5 and
RA3.0 were 2.4 times and 2.9 times larger, respectively. There were no significant differences in P. gingivalis.
These results suggest that the bacterial attachment might be affected by the difference of implant surface
characteristics and surface roughness.
