Biophysics and Physicobiology
Online ISSN : 2189-4779
Regular Article
Fluorescent nanodiamonds as a robust temperature sensor inside a single cell
Takeharu SekiguchiShingo SotomaYoshie Harada
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2018 Volume 15 Pages 229-234

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Abstract

Thermometers play an important role to study the biological significance of temperature. Fluorescent nanodiamonds (FNDs) with negatively-charged nitrogen-­vacancy centers, a novel type of fluorescence-based temperature sensor, have physicochemical inertness, low cytotoxicity, extremely stable fluorescence, and unique magneto-­optical properties that allow us to measure the temperature at the nanoscale level inside single cells. Here, we demonstrate that the thermosensing ability of FNDs is hardly influenced by environmental factors, such as pH, ion concentration, viscosity, molecular interaction, and organic solvent. This robustness renders FNDs reliable thermom­e­ters even under complex biological cellular environment. Moreover, the simple protocol developed here for measuring the absolute temperature inside a single cell using a single FND enables successful temperature measurement in a cell with an accuracy better than ±1°C.

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© 2018 THE BIOPHYSICAL SOCIETY OF JAPAN
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