2014 Volume 61 Issue 1 Pages 50-54
Holography is an optical technique that can record three dimensional information of a sampling space in its entirety with information on the structure of objects and their location within the space. The first use of an underwater holographic camera for observations of planktonic organisms was in 1970. Underwater digital Holographic cameras, such as LISST-HOLO (Sequoia Scientific, Inc.) are now commercially available. The resolution and characteristics of reconstructed images of planktonic organisms and artificial aggregates obtained by LISST-HOLO were examined in the present study. Resolution of reconstructed images depended on the area of the hologram for their calculation. Reconstructed images showed sharp outlines of objects but thin slices could not display the inner structure of them. The underwater digital Holographic camera, LISST-HOLO, could be a powerful tool for observations on the distribution of micro- and meso-plankton when they are more abundant than 10–100 individuals L－1.
