Accumulation of cadmium in the liver and kidney following a long term period of oral administration of cadmium in mice

—Detection of tissue cadmium by in-air micro-PIXE system—

Abstract

ICR female mice were allowed free access to water to which Cd (CdCl₂) was added at levels of 100mg/l. Mice were sacrificed and liver and kidney were removed immediately after 18 and 24 months. Three μm paraffin sections of the organs were prepared for HE stain and immunohistochemical stain for metallothionein (MT). Ten μm frozen sections of the organs were prepared for detection of Cd using In-air micro-PIXE system. Histological changes and MT expression were observed in the liver cells and the renal proximal tubular epithelium after Cd administration for 18 and 24 months. Using in-air micro-PIXE, Cd accumulation in the liver and kidney were detected in the mouse administratied Ca for 24 months but not detected in the mouse administratied Ca for 18 months.