Biological Sciences in Space
Online ISSN : 1349-967X
Print ISSN : 0914-9201
ISSN-L : 0914-9201
Enhanced Expression of The LDH-A Gene after Gravity-changing Stress in Human RSa Cells
Tetsuhiro MoriyaKazuko KitaShigeru SugayaChieko WanoNobuo Suzuki
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2002 Volume 16 Issue 1 Pages 12-17

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Abstract

A major issue in radiation and space biology is whether gene expression levels are altered in cells exposed to gravity-changing stress. In the present study, genes up- or down-regulated in radiation-sensitive human RSa cells cultured under gravity-changing conditions, were identified using a PCR-based mRNA differential display method. Exposure of cells to gravity-changing stress was performed by free-fall with a drop-shaft facility or by an airplane-conducted parabolic flight. Among the candidates for gravity-changing stress-responsive genes obtained by the differential display analysis, the lactate dehydrogenase A gene (LDH-A) was confirmed by Northern blotting analysis to exhibit increased expression levels. The gravity-changing stress consisted of a combination of microgravity and hypergravity. However, exposure of the cells to hypergravity produced by centrifuge only slightly affected the LDH-A mRNA expression. Thus, LDH-A was found to be a candidate for the genes which play a role in the cellular response to gravity-changing stress, and mainly to microgravity.

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© 2002 by Japanese Society for Biological Sciences in Space
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