Optical microscopy of Arabidopsis seedlings fixed in non-fresh FAA using Kennedy Fixation Tubes (1)

Yoshihiro Honma; Izumi Nakabayashi; Daisuke Tamaoki; Haruo Kasahara; Noriaki Ishioka; Toru Shimazu; Hirokazu Kasahara; Mitsuhiro Yamada; Ichirou Karahara; Seiichiro Kamisaka

doi:10.2187/bss.17.307

Yoshihiro Honma, Izumi Nakabayashi, Daisuke Tamaoki, Haruo Kasahara, Noriaki Ishioka, Toru Shimazu, Hirokazu Kasahara, Mitsuhiro Yamada, Ichirou Karahara, Seiichiro Kamisaka

Author information

Yoshihiro Honma
Department of Biology, Toyama University
Izumi Nakabayashi
Department of Biology, Toyama University
Daisuke Tamaoki
Department of Biology, Toyama University
Haruo Kasahara
Japan Manned Space Systems Corporation
Noriaki Ishioka
Japan Aerospace Exploration Agency
Toru Shimazu
Japan Space Forum
Hirokazu Kasahara
Hokkaido Tokai University
Mitsuhiro Yamada
The University of the Air
Ichirou Karahara
Department of Biology, Toyama University
Seiichiro Kamisaka
Department of Biology, Toyama University

JOURNAL FREE ACCESS

2003 Volume 17 Issue 4 Pages 307-308

DOI https://doi.org/10.2187/bss.17.307

Details

Abstract

Samples will be kept in non-fresh fixative for longer period than usual in Kennedy Space Center Fixation Tubes (KFT) when they will be fixed for microscopy in the International Space Station (ISS). It is necessary to examine characteristics of samples prepared under such conditions and to improve preservation of the samples. In this study, morphology of Arabidopsis tissues prepared under such conditions was examined under a light microscope. Deformation of cell shape was observed in tissues with well-developed intercellular spaces when they were fixed in non-fresh fixative for longer period in KFTs.

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