BioScience Trends
Online ISSN : 1881-7823
Print ISSN : 1881-7815
ISSN-L : 1881-7815
Brief Report
A novel EGFP-expressing nude mice with complete loss of lymphocytes and NK cells to study tumor-host interactions
Kumiko GotohRyusho KariyaKouki MatsudaShinichiro HattoriKulthida VaeteewoottacharnSeiji Okada
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2014 Volume 8 Issue 4 Pages 202-205


Enhanced green fluorescent protein (EGFP) expressing Balb/c nude mice strain with Rag-2 and Jak3 double mutants (Nude-R/J-EGFP mice) was established to improve the take rate of human tumors and to distinguish tumor and host cells. EGFP was ubiquitously expressed in all organs including the brain, lung, liver, heart, kidney, spleen, and gastrointestinal tract in Nude-R/J-EGFP mice. The mice showed complete loss of T lymphocytes, B lymphocytes, and NK cells, indicating a higher take rate of human tumor xenograft. M213-mCherry, an mCherry expressing the cholangiocarcinoma cell line, was successfully detected and tumor vessels derived from the host were clearly identified with fluorescence imager. Thus, dual-color fluorescence imaging visualizes the tumor-host interaction by non-invasive in vivo fluorescent imaging in Nude-R/J-EGFP mice. These finding suggests that Nude-R/J-EGFP mice are becoming a powerful tool to investigate human tumor-host interactions.

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© 2014 International Research and Cooperation Association for Bio & Socio-Sciences Advancement
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