Abstract
A method is described whereby carboxyhemoglobin in the blood is rapidly and accurately determined by spectrophotometric analysis. The γ-wave ranging from 400 to 500 nm was used for the determination, and to increase the stability of the absorption value the isosbestic point was utilized. This method is simple; a heparinized whole blood sample, diluted to about 1:100 to 1:200, is placed in a cuvette and the absorbance is measured at the wavelengths of 413.2 nm, 421 nm and 431.3 nm against water as the zero reference standard. The absorbances of the standard solutions of 0% of CO-Hb and 100% of CO-Hb are also measured, and by these absorbance values the concentrations of CO-Hb are calculated. To make the procedure simpler, it is convenient to use a factor composed by personal and instrumental ones, which is obtained during the calibration by the spectrophotometer. Using this factor, the determinations of the absorbances of standard solutions of 0% and 100% of CO-Hb can be omitted.
