2000 Volume 49 Issue 3 Pages 135-159
Chemiluminescence(CL)is the emission of light as the result of electronic excitation of the luminescing species by a chemical reaction of precursor of that species.Many CL mechanisms have been studied and utilized to analyze either the chemiluminogen or other compounds that participate in the CL reaction.Generally, the CL detection methods are known to be highly sensitive and selective.Thus, various kinds of CL reagents, such as luminols, acridinium esters, aryloxalates, dioxetanes, and Ru(bpy)32+, have been developed and their properties as chemiluminogens examined.Enhancement of the CL reactions has also been examined, and several substances such as 4-iodophenol for luminol-peroxidase-hydrogen peroxide CL, pyrimido[5, 4-d]pyrimidines for peroxyoxalate CL and quinine for cerium(IV)CL were found to be useful for this aim.During the last couple of decades, many procedures utilizing CL detection have been developed for determining trace amounts of organic and inorganic compounds, including some of which are biologically important.Among the methods, HPLC or immunoassay with CL detection is more popular and applicable in such fields as clinical and environmental chemistry.Various compounds of biological importance have been determined as diagnosis markers.More recently, capillary electrophoresis with CL detection has become a preferred tool for the analysis of biological samples available in small quantities.In this paper, the application and usage of CL detection in a variety of instrumental analyses, with special emphasis on HPLC, is discussed.
