Screening method for serum γ-globulin concentration using a sulfosalicylic acid solution containing Triton X-100 as a precipitant

Abstract

The reaction of a 30g/l sulfosalicylic acid solution containing Triton X-100 with serum protein has been investigated.The result indicated that this reagent has an ability for protein fractionation, and was thought to precipitate the serum γ-globulin in the concentration region of about more than 70g/l Triton X-100.The measurement values obtained by this reagent containing 70g/l Triton X-100 correlated well with the serum γ-globulin concentrations by the conventional method combining the biuret method and cellulose acetate electrophoresis(r=0.917).It seems that this reagent can be used for screening the serum γ-globulin concentration.