2002 Volume 51 Issue 12 Pages 1179-1182
Multilayer films composed of poly(ethyleneime) (PEI) and uricase were prepared by an alternate deposition of PEI and uricase on the surface of a platinum (Pt) electrode for developing amperometric uric acid sensors. PEI and uricase were successfully built into a multilayer film, and the loading of uricase in the film was evaluated using a quartz-crystal microbalance. The Pt electrode coated with the PEI/uricase multilayer film functioned as an amperometric biosensor senstive to uric acid. The output current of the sensor depended linearly on the number of PEI/uricase layers in the film.