2002 Volume 51 Issue 4 Pages 205-233
Chemiluminescence is a phenomenon of light emission during the course of a chemical reaction. The chemiluminescent method of analysis has received much attention due to its low detection limits and wide linear dynamic ranges, with relatively simple instrumentation. In recent years, further progress has been made in developing the chemiluminescent method for the quantitative analysis of inorganic, organic and biological substances. In order to increase the sensitivity of the CL method, micelles, reverse micelles and emulsions have been examined as solubilization media or intensity amplification agents. Since chemiluminescent detection is now one of the most sensitive detection methods in analytical chemistry, it is widely utilized in a variety of analytical methods, such as flow-injection analysis, high-performance liquid chromatography, capillary electrophoresis and immunoassay. Chemiluminescence biosensors combining substrate-specific enzyme reactions and chemiluminescence for biological substanses have also been extensively studied because they unite high selectivity and high sensitivity. This paper reviews the recent developments in the analytical applications of chemiluminescence.
