Abstract
The retention behavior of the reversed phase was evaluated under 100% aqueous conditions. It is commonly said that reversed phases, such as ODS (C18) and C8, show a decrease of the retention time under 100% aqueous conditions. It was previously reported that the mechanism of the decrease in retention was found to be that the 100% aqueous mobile phase was expelled from the pore of the packing materials, so that the stationary phase in contact with the mobile phase decreased. In this study, the wettability between the C18 stationary phase and water as a mobile phase was analyzed. It was concluded that the retention behavior could explained by capillarity, and reversed-phase separation could be carried out under 100% aqueous conditions, even if a mobile phase can not wet the stationary phase.
