固定化ヒスタミンオキシダーゼを用いる吸光検出フローインジェクション分析による魚醤中のヒスタミンの定量

渡辺 瞬; 松尾 謙一; 鈴木 保任; 橘 正樹; 谷 和江; 小泉 均; 松本 邦男; 木羽 信敏

doi:10.2116/bunsekikagaku.56.1033

Research Papers

Determination of Histamine in Fish Sauce by Photometric Flow Injection Analysis with Immobilized Histamine Oxidase Reactor

Shun Watanabe, Kenichi Matsuo, Yasutada Suzuki, Masaki Tachibana, Kazue Tani, Hitoshi Koizumi, Kunio Matsumoto, Nobutoshi Kiba

Author information

Shun Watanabe
Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
Kenichi Matsuo
Department of Applied Bioscience, Faculty of Engineering, Kanagawa Institute of Technology
Yasutada Suzuki
Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
Masaki Tachibana
Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
Kazue Tani
Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
Hitoshi Koizumi
Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
Kunio Matsumoto
Department of Applied Bioscience, Faculty of Engineering, Kanagawa Institute of Technology
Nobutoshi Kiba
Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

Keywords: histamine, FIA, histamine oxidase, photometric detection, HACCP

JOURNAL FREE ACCESS

2007 Volume 56 Issue 12 Pages 1033-1036

DOI https://doi.org/10.2116/bunsekikagaku.56.1033

Details

Abstract

Histamine in fish sauce was rapidly determined with a single-line flow-injection system having an immobilized histamine oxidase/peroxidase reactor. Hydrogen peroxide produced by histamine oxidase (EC1.4.3.-) reaction was monitored photometrically by using N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methoxyaniline and 4-aminoantipyrine as color reagents with peroxidase (EC 1.11.1.7) at 530 nm. The carrier solution was 0.1 M CHES-NaOH buffer (pH 8.0). The reactor was stable for 2 weeks. The detection limit was 3 μM and the range of determination was 10∼1000 μM. The sample throughput was about 20/h without any carryover. The results showed a good correlation with those obtained using a conventional HPLC method.

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