Quantitative Analysis for Water-soluble Porphyrin Derivatives Adsorbed in Saccharomyces cerevisiae by Absorption Spectrophotometry Using Confocal Laser Scanning Microscope

Abstract

An evaluation of the bio-affinity of methoxo(alkyloxo)- tetraphenylporphyrinatoantimony bromide (1) was examined by an absorption experiment of 1 toward Saccharomyces cerevisiae. An aqueous solution of 1 was added to an aqueous solution of S. cerevisiae. Then an aqueous solution of agar was added into the solution in order to stop the Brownian motion of bacteria during analysis. A prepared aqueous solution containing 1(10 μmol dm⁻³), S. cerevisiae (1.0×10⁴ cells cm⁻³) and agar (0.4 wt%) was taken inside a space surrounded by a silicone spacer (1 cm×1 cm, thickness 50 μm) put on a slide glass, which was set on a confocal laser scanning microscope (CLSM) stage. By measuring the diameter of the cell and the absorbance on CLSM, the concentrations of 1 adsorbed inside S. cerevisia were determined to be 54.2±7.3 for 1a, 49.8±6.2 for 1b, and 22.5±15.5 mmol dm⁻³ for 1c according to Lambert-Beer`s law.