A Simple Method for Transferring DNA from DNA Printed Matter

Abstract

We developed a simple electrochemical device for transferring DNA (deoxyribonucleic acid) from DNA printed matter onto filter paper (5 mm×10 mm) without spoiling the DNA printed matter. The device is composed of a pair of pin-shaped electrodes, an electrode stand and a compact stage. Extracted DNA from the filter paper was amplified by PCR (polymerase chain reaction), and detected. Various transferring conditions (i.e., kinds of moisture solvent, applied voltage and contact time) were optimized. We confirmed that an excess voltage and a longer contact time caused the decomposition of DNA. When we used TBE buffer (Tris-Borate EDTA) as a moisture solvent, the optimum applied voltage was 10 V, and the contact time was 60 seconds.