Abstract
Nuclear magnetic resonance (NMR) spectra have mainly been used for qualitative analysis, such as molecular structure analysis. In the present work, the analytical parameters were considered to apply to accurate quantitative analysis. In optimizing the analytical parameter, the influences for results on baseline correction and integral range were considered. In this result, optimized analytical conditions for quantitative NMR were determined. The purity of 17β-estradiol (NMIJ CRM) was measured under optimized conditions. The result of a purity analysis corresponded within the range of a certified value, and verified the use of NMR for accurate quantitative analysis.
