Development of a Chemiluminescence Analysis System Using a Microfluidic Device Capable of Autonomous Liquid Transfer and an Organic Photodiode Detector

Abstract

A novel chemiluminescence analysis system using a polydimethylsiloxane (PDMS) microfluidic device and an organic photodiode (OPD) detector was developed. The sample and reagent solutions in the reservoir on the degassed PDMS microfluidic device were autonomously flowed into the microchannel in the PDMS microfluidic device, since the degassed PDMS resin absorbs the air in the microchannel. The OPD was fabricated by a spin-coating method using poly(3-hexylthiophene-2,5-diyl) and [6,6]-phenyl-C61 butyric acid methyl ester as a material of photoelectric conversion layer. The measurement of hydrogen peroxide was evaluated using the chemiluminescence analysis system. SuperSignal^TM ELISA Femto Substrate and Horseradish Peroxidase were used in the measurement. The calibration curve for hydrogen peroxide was linear for concentrations under 3.2 mM with a correlation coefficient of 0.9986. The limit of detection, defined as three-times the standard deviation of the blank signal, was estimated to be 4.4 μM. Compared to the hydrogen peroxide assay kit using a 96-well microtiter plate and a micro plate reader, the amount of sample and reagent consumption and the analysis time could be reduced to 1/5 and 1/10, respectively. The chemiluminescence analysis system was successfully used in the determination of hydrogen peroxide in a commercially available disinfectant, oxydol. The chemiluminescence analysis system would be useful for on-site environmental measurement, quality control of food, point-of-care testing etc. since a pump is unnecessary and the detector is very small and lightweight.