Purification of 2-Aminobenzamide Labeled Glycans Using Monolithic Solid-phase Extraction Centrifugal Columns

Abstract

Glycans have important functions in living organisms. In the analysis of glycans, fluorescence derivatization is followed by separation analysis using high-performance liquid chromatography. It is necessary to remove excess unreacted derivatization reagent. In this study, we investigated the selective extraction of 2-aminobenzamide labelled glycans using a monolithic silica disk-packed spin column, MonoSpin column, in order to simplify this pretreatment. MonoSpin Amide and MonoSpin NH2 columns, which have amide and amino groups as functional groups, respectively, showed the same efficiency as the conventional solid-phase extraction method in the removal of 2-AB and the recovery of labeled glycan samples. The MonoSpin column is expected to be applied to high-throughput glycan sample preparation since it can be easily pretreated only by centrifugation.