Preliminary Study for Discrimination of Raw Material Beans in White Bean Paste by SDS-PAGE

Abstract

We verified the possibility of discrimination of raw material beans in white bean paste by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) of the protein fractions of white bean paste made from tebo (white kidney beans), butter beans, and baby lima beans, which are the raw material beans used for white bean paste in Japan. We found some characteristic bands in each of the white bean paste samples of tebo, butter beans, and baby lima beans. In addition, those characteristic bands were detected in simulated white bean paste in which the mixing ratio of each ingredient was varied. Although it is necessary to evaluate the usefulness of these markers based on the results of quantitative and statistical analyses in future work, these results suggest that SDS-PAGE could be a potential tool for discriminating the raw material beans of white bean paste.