Abstract
The gas chromatography was applied for the qualitative identification of benzyl-, phenoxy-methyl-, phenoxyethyl-, phenoxypropyl-, 2, 6-dimethoxyphenyl-, 5-methyl-3-phenyl-4-isoxazolyl-, and 5-methyl-3-chloropheny1-4-isoxazolyl penicillins. A few mg samples were heated with 2 ml of 20% sodium hydroxide for about 30 min. for their hydrolysis.
The organic acids derived from side chaines were converted to their methyl esters, and gas chromatographically separated using a 1.5 m column of 3.5% SE-30. A connected SE-30 and QF-1 column was also applied for the separation of the esters with closely resembling retention times.
