Abstract
The higher polarity of free fatty acids often hinders their gas chromatographic determination by their adsorption of the column packings and walls, injection and connection tubes. They also disturb the analysis with their frequent association.
The authors selected the solid support and stationary liquid together with investigation on the silane treatment and the addition of phosphoric acid to avoid the above difficulties, and employed glass beads as the optimum solid support and diethylene glycol adipate polyester as the stationary liquid. They also recommended that every passage of the gas should be constructed with borosilicate glass. By this means, saturated and unsaturated free fatty acids of C5C22 were separated and determined on reproducible and symmetric peaks under the column temperature below 200°C
