Determination of Cellocidin by spectrophotometric method

Determination of Cellocidin in fungicide formulations. I

Abstract

A spectrophotometric method for the estimation of Cellocidin, acetylenedicarboxamide, based on the addition of ammonia to it was presented. Two hundred milligrams of 10% wet table powder was weighed into a 500 ml volumetric flask, and 400 ml of water and 5 ml of 1 N hydrochloric acid were added. The flask was warmed for 10 minutes with occasional shakings in a water bath held at about 70°C and allowed to cool. The suspension was made up to 500 ml with water and filtered through a dry filter paper. A 5 ml portion ofthe filtrate was pipeted into a 100 ml Erlenmeyer flask and 5 ml of 1.2 N ammonia water was added. The flask was connected with a Liebig condenser and placed for 20 minutes on a boiling water bath. After standing to cool, the content of the flask was transfered into a 50 ml volumetric flask and made up to 50 ml with water. The absorbancy of the solution at 296 mμ was measured using a cell with 10 mm width. The “blank” obtained by the way mentioned below was subtracted from the absorbancy, and the amount of Cellocidin was calculated from the calibration curve prepared by treating the standard solution containing 80400 μg of Cellocidin through the whole procedure.
“The blank” : Five ml of the filtrate of the sample suspension was pipeted into a 50 ml volumetric flask.Forty of water and 5 ml of 1.2 N ammonia water were added and it was immediately made up to 50 ml with water. Within two minutes after the addition of ammonia, the absorbancy of the solution at 296 mμ was measured.
The raw materials and by-products of the Cellocidin synthesis did not interfere with the analysis. The method was applicable to the analysis of the formulations containing other fungicides, phenylmercuric acetate or dithianone.
The compound obtained by treating Cellocidin with ammonia was identified with 1-amino-1, 2-ethylenedicarboxamide by IR and UV spectra.