Gas chromatography of triglycerides; Scrutinization of determination

Abstract

Operating conditions in gas chromatography of triglycerides were investigated. Liquid phase OV-17 having a high thermal stability gave an excellent separation of triglycerides. The result by using OV-17 revealed the differences in the constituent triglycerides of butter and margarine. Better column packings were obtained by filtration method than by evaporation method. The regulation system for carrier gas with mass flow controllers improved the accuracy of quantitative determination of glycerides.
Lard, beaf tallow, whale oil, butter, margarine and cheese were analysed for their triglycerides, with a 35 cm glass column packing with 2% OV-17 on 80100 mesh Shimalite W, by programmed temperature gas chromatography between 200°C and 310°C.