Separation of sugar phosphates on a cellulose ion exchanger

Abstract

A chromatographic separation of sugars and their phosphates such as fructose, glucose, glucose-1, glucose-6-, fructose-6-phosphate and fructose-1, 6-diphosphate has been studied.
A column of 250 mm×13 mmφ packed with DEAE cellulose of borate form gave an efficient separation, when glucose and fructose were eluted through the column with 0.015 M sodium tetraborate, subsequentely glucose-1-phosphate with a mixture of 0.010 M sodium tetraborate and 0.025 M ammonium chloride (1:1), glucose-6-phosphate with 0.04 M ammonium chloride, fructose-6-phosphate with a mixture of 0.02 M sodium chloride and 0.02 M hydrochloric acid (1 :1), and finally fructose-1, 6-diphosphate with a mixture of 0.02 M sodium chloride and 0.06 M hydrochloric acid (1:1).
The eluate concentration was determined by anthrone method.
The elution behaviors of such sugars and their phosphates on DEAE Sephadex and ECTEOLA cellulose, together with their adsorption isotherms, have also been given.