Determination of skin sugar

Abstract

Relationship between Diabetes mellitus and blood sugar have been discussed very often, but only few reports have been given concerning the relationship of Diabetes mellitus and organ sugar, especially skin sugar. For this purpose, a small amount of living skin must be subjected to a chemical investigation.
The informations available at present in literatures are insufficient since they depend on the macro method using more than 50 mg of skin. The object of the present investigation is to determine skin sugar clinically with a higher accuracy, and the authors have established a micro procedure of skin sugar determination by glucose-oxidase method, which is applicable to about 10 mg or less of skin sample.
The methods and technics for taking skin samples of rat, guinea pig and human, and the procedure for determination of their glucose contents are given in detail.
The authors have employed a new method for pretreatment of skin involving cold infusion of refrigerated pieces of skin specimens more conveniently than the conventional homogenation.