Abstract
A crystalline precipitate of Cu(II)-5'-adenylic acid (5'-AMP) was isolated from the solution of pH 4.04.5 by heating at 40°C. An elementary analysis showed that it had a composition of [(C10H12N5O7P)Cu(H2O)].Chemical structure of this complex was discussed by infrared absorption spectra in comparison with those of 5'-AMP and 5'-AMP-2Na. Shift of C=N+ of 1690 cm-1 to 1660 cm-1 and a little change in the band at 1100 cm-1 (phosphate groups) upon complexation showed that N1 of adenine ring moiety and also phos-phate group of 5'-AMP are the site of coordination. Existence of the coordination bonding between Cu(II) and N1 of adenine ring moiety was confirmed by the fact that N1-methyl-5'-AMP did not form insoluble Cu(II) complex in an acid solution. Additional experiments to get insoluble Cu(II) complexes of ADP and ATP in the same condition as 5'-AMP did not succeed. Separation of 5'-AMP from other adenine nucleotides may be possible by the use of the complexation with Cu(II).
