Abstract
Analytical methods have been developed for thedetermination of Salithion (2-methoxy-4H-1, 3, 2- benzoxyphosphorin-2-sulfide) by using thin layer chromatography and gas chromatography. An acetone solution of Salithion was applied to silica gel plate, developed with n-hexane-benzene (1+1), eluted with methanol and heated for 15minutes at 4555°C with added sodium hydroxide solution. Saligenin produced was then colored with potassium ferricyanide and 4-aminoantipyrine at pH 8.2 and spectrophotometrically determined from its absorbance at 505mμ.
Alternatively, gas chromatographic determination of Salithion was performed on a gas chromatograph equipped with a hydrogen flame ionization detector. The operating condition was as follows. Column: 1.5m×3mm made of pyrex; column package:6080 mesh, acid-washed, silane-treated Chromosorb W coated with 2% LAC-2R-446; column temperature: 165°C; carrier gas: nitrogen 25ml/min; and internal standard: benzylbenzoate.
The analytical results by both methods agreed on technical products of Salithion. Standard deviations for TLC method and GC method were 0.32% and 0.80%, respectively. The latter was more simple and rapid.
