1978 Volume 27 Issue 12 Pages 753-758
The rapid, sensitive and specific high-speed liquid chromatographic method for the quantitative analysis of antitussive, expectorant and antihistaminic in anticold preparations was established. Noscapine was separated by injection of methanol solution to a column (50 cm × 3 φ ID) packed with polymethylmethacriratestyrene-divinylbenzene copolymer (1:1, MCL-DVB). Dextromethorphane HBr was separated by injection of methanol solution to a column (50 cm × 3 φ ID) packed with MCL-DVB(1 : 2). Alimemazine tartrate, prome-thazine methylenedisalicylate and isothipendyl HCl were prepared by injection of methanol solution to a column (50 cm × 3φ ID) packed with hydroxymethylated MCL-DVB. Noscapine, dextromethorphane, alimemazine, promethazine and isothipendyl were eluted with mixture of methanol and 28% ammonia-water (99:1) and detected at 220 nm, 227 nm and 254 nm. Other ingredient such as acetaminophen, phenacetin, ethoxybenzamide, caffeine and methylephedrine HCl, did not interfere with the analysis. The method is sensitive and accurate for analysis of antitussive, expectorant and antihistaminic in anti-cold preparations.
