Abstract
It was found that the magnitude of electrokinetic (zeta-) potentials between cellulose thin-layer and basic mobile phase, and that of electronic charge density on center atom of inorganic oxyanions were the main controlling factor for thin-layer chromatographic separation of inorganic oxyanions. Organic esters such as steroidal sulfates (cholesteryl 3-O-sulfate, dehydroisoandrosterone 3-O-sulfate) and nitrate (6-nitro-androstenediol diacetate), sugar sulfates (6-monosulfate and 1, 6-disulfate of glucose, galactose and fructose) and sugar phosphates (1-monophosphate, 6-monophosphate and 1, 6-diphosphate of glucose and fructose) have the same chromatographic characteristics as the inorganic oxyanions. In the anion exchange chromatography of adenosine phosphates (5'-AMP, 5'-ADP, 5'-ATP), the phenomena is more evident than in the partition chromatography of the inorganic or organic anions described above. When compared with the original free compounds, hRf values of these organic esters were rather small and it was recognized that the additivity between substituent number (n=13) of sulfate and phosphate residues and difference in hRf values was set up. In the separation of organic oxyanions, the original mobility based on the organic functional groups was also superimposable on that of phosphate or sulfate residues.
