1979 Volume 28 Issue 11 Pages 671-675
As new method of derivatization connected with HPLC, the rapid derivatization using thermal-reaction column is described. The outline of this as follows. Aqueous solution aldehyde is injected to thermal-reaction column that has been packed with 2, 4 dinitrophenylhydrazine supported on Celite 545 {(80100) mesh} and heated at 100°C. Then, thermal-reaction column is connected to HPLC instrumentusing 6-way valve and the thermal reaction products (2, 4-dinitrophenylhydrazone, etc.) are transferred by mobile phase (H2O-MeOH, 40/60) into the analytical column packed with ODS-silica, i.d. 4mm, length 25cm. They were separated to peaks of each components and detected at 350 nm which is specific absorption of 2, 4-DNPH. The method of quantitation is peak-area calibration procedure using disc integrator. As a result, it was found that the thermal-reaction proceeded quantitatively. Therefore, it was possible to determine amounts of 10 ng aldehyde rapidly and simply. As a practical example, we applied this method to the quantitative analysis of acetaldehyde in wines.
