Simple and rapid determination of Fenitrothion in water by reversed phase adsorption liquid chromatography

Abstract

Reversed phase adsorption high pressure liquid chromatography was examined to develop a simple and rapid method for the determination of Fenitrothion {O, O-dimethyl (3-methyl-4-nitrophenyl) phosphorotioate} in water. A diphenylsilane chemically bonded glass beads column was found to be useful for the adsorption of Fenitrothion in water. Fenitrothion is adsorbed from sample water directly injected on the diphenylsilane bonded beads and eluted as a sharp peak by a linear gradient elution from water to 100% acetonitrile. The peak height detected at 280 nm was proportional to the amount injected in the range from 0 to 800 ng. The recovery of Fenitrothion from fortified distilled water and pond water at sub-ppb level was (97.8±0.25)% and (95.6±1.10)%, respectively.