1979 Volume 28 Issue 6 Pages 337-340
As a new method of thin layer chromatography (TLC), a thermal reaction thin layer chromatography for the rapid analysis of aromatic amines which were present in synthetic rubber as anti-oxydants is described. The outline of thermal reaction TLC method is as follows. The mixture of 2, 4-dinitrofluorobenzene supported on Celite 545 and synthetic rubber is placed in a glass tube and the temperature is raised from 50 to 300°C at the rate of 4°C/min. The thermal reaction products are carried on by carrier gas (N2-NH3 mixed) and condensed on a TLC plate (20 × 20 cm) which moves slowly as correspond to the temperature raising of furnace. The plate is then developed with petroleum-ether/ethylacetate (4/1, v/v), giving the chromatogram of 2, 4-dinitrophenyl derivatives of amines (2, 4-DNP). The both chromatograms of the 2, 4-DNP of amines obtained from synthetic rubber and standard sample were identical. Therefore, it is possible to measure qualitatively the amines present in synthetic rubber. In addition to this, it was found that the thermal reaction proceeded quantitatively.
