Abstract
Four kinds of the immobilized matrixes (Nylon 66 tube, Nylon 6 polymerized in glass capillary, whisker-walled glass capillary and controlled pore glass beads) were used for the fundamental consideration of the application to reaction detector and discussed about their characteristics. Triethyl oxonium tetrafluoroborate was used for O-alkylation of nylon tube and nylon was further reacted with 1, 6-diaminohexane as spacer. Glucose oxidase (GOD) and cholesterol oxidase (COD) were immobilized on the nylon through bismidate I. (Nylon 66, inner diameter φ: 0.2 mm, immobilized enzyme: GOD, activity: 0.53 μmol min-1 m-1), (Nylon 66, φ : 0.2 mm, COD, 0.43 μmol min-1 m-1), (Nylon 6 polymerized in glass capillary, φ: 0.7 mm, GOD, 1.68 μmol min-1 m-1). Whisker-walled glass capillary which was made by fluoroether was reacted with dimethyl dichloro silane. COD was immobilized on it through ethylenediamine and glutalaldehyde. (Whisker-walled glass capillary, φ : 0.26 mm, COD, 3.37 μmol min-1 m-1), (glass beads, GOD, 40.5 μmol min-1 g-1), (glass beads, COD, 31.9μmol min-1 g-1). The activities of these immobilized enzyme were measured by 4-aminoantipyrinephenol method in the presence of peroxidase. As the results, nylon tube supported enzyme had lower activity compared with other glass matrixes, but it was easier to handle than glass capillary column. Whisker-walled glass capillary column was a good matrix for immobilized enzyme as well as controlled pore glass beads because of the large surface area and of the high enzyme activity. A glass capillary (40 cm) on which enzyme was immobilized showed reduced band broadening compared with 10 cm bed reactor of glass beads packed teflon tube (φ: 1.5 mm)
