BUNSEKI KAGAKU
Print ISSN : 0525-1931
POST-COLUMN FLUOROMETRIC DETECTION OF REDUCING SUGARS IN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY USING ARGININE
Hirohisa MIKAMI, Yasuo ISHIDA
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Keywords: high performance liquid chromatography, fluorometric detection of reducing sugars, arginine as detection reagent
JOURNAL FREE ACCESS

1983 Volume 32 Issue 6 Pages E207-E210

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Abstract
Arginine was found to react with reducing sugars after being heated in boric acid solution at neutral pH; highly fluorescent derivatives were produced. This reaction was applied to a postcolumn reaction system for high performance liquid chromatography and found to be useful for highly sensitive determination of reducing sugars.
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© The Japan Society for Analytical Chemistry
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