Gas-liquid chromatographic analysis of alditol acetates

Preparation of heat-stable, weakly polar columns by superposing two different packings and application to the separation of partially methylated alditol acetates

Abstract

We prepared heat-stable support of appropriate polarity by superposing two packings (SE30 and Silar 10C) in one column. Relative retention times of 8 partially methylated alditol acetates from arabinose, 8 from xylose, 17 from glucose, 23 from galactose, and 16 from mannose, were measured at various preparations of bed lengths, and the results indicated suitability of these column for the separation and indentification of these partially methylated alditol acetates.