High-performance liquid chromatographic determination of arachidonic acid and eichosapentaenoic acid with 9-aminophenanthrene as a fluorescence prelabeling agent

Abstract

A procedure for high-performance liquid chromatographic (HPLC) determination of picomole levels of arachidonic acid (AR) and eichosapentaenoic acid (EPA) has been developed by using 9-aminophenanthrene as a fluorescence prelabeling reagent. The correlation coefficients between the proposed method and a routine gas chromatographic and a UV labeling HPLC methods were determined. An adequate correlation was observed between the present method and the other two methods. The recovery test of AR and EPA added to a human plasma for the proposed method showed the satisfactory results for both fatty acids.