HIGH PERFORMANCE ION EXCHANGE CHROMATOGRAPHY OF PROTEINS

Toshihiko KADOYA; Yasuhiko AMANO; Toshiaki ISOBE; Yoshio KATO; Koji NAKAMURA; Tsuneo OKUYAMA

doi:10.2116/bunsekikagaku.33.7_E287

Toshihiko KADOYA, Yasuhiko AMANO, Toshiaki ISOBE, Yoshio KATO, Koji NAKAMURA, Tsuneo OKUYAMA

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Keywords: high performance ion exchange chromatography of proteins using aqueous solvent, relation between retention times and isoelectric points of protein, preservation of biological activity of proteins

JOURNAL FREE ACCESS

1984 Volume 33 Issue 7 Pages E287-E294

DOI https://doi.org/10.2116/bunsekikagaku.33.7_E287

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Abstract

High performance ion exchange chromatography using aqueous solvents is presented for the separation of proteins. Diethylaminoethyl(DEAE) resin for the anion exchange column and sulfopropyl(SP) resin for the cation exchange column were utilized. Proteins, having a molecular weight of 10000 - 190000 daltons and an isoelectric point of 3.9-10.7, were separated within 60 min by sodium chloride linear gradient elution. The protein was eluted according to its isoelectric point. These columns are capable of separating proteins with the biological activity in high speed, high resolution, large capacity and considerably high recovery.

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