1985 Volume 34 Issue 1 Pages 16-20
This procedure can be carried out in aqueous or water-methanol medium. In aqueous media, the Besalicylidene-2-aminophenol (SAPH) complex is quantitatively formed in the range of pH 8.18.6. This complex has an excitation max. at 345 nm and fluorescence max. at 440 nm. The fluorescence intensity is enhanced remarkably under the addition of ethylenediamine (en) as a buffer with 1 ml of 0.04 % SAPH, 0.0054 μg Be/25 ml can be determined in the presence of 1 ml of en. The relative standard deviation(R. S. D.) was 3.5 % for 0.019 μg beryllium. In the water-methanol media, the formation of the complex at pH 10 is highly reproducible. The complex is stood for 25 min before measurements. Here, 0.014 μg Be/25 ml can be determined. The R. S. D. was the same as the case of aqueous media. The Be complex was more stable in the water-methanol media than in the aqueous media. The fluorescence of the complex formed in the mixed medium (H2O : MeOH=2 : 3) keeps the intensity constant at least for 30 min. Although cobalt, chromium, and others interfere with the determination, they can be masked with EDTA. The proposed procedure was applied to the analysis of berylliumcopper alloy.
