1985 Volume 34 Issue 1 Pages 55-58
Trace amounts of aliphatic aldehydes reacted with acetylacetone and ammonium acetate to give its 1, 4-dihydrolutidine derivatives. The separation of derivatives from each aldehyde was investigated by high performance liquid chromatography equipped with a fluorescence detector and completely achieved under the operating conditions as follows. Column was 25 cm×4.6 mm stainless steel tube packed with Zorbax ODS, mobile phase was 50 % MeOH and its flow rate was 0.8 ml/min. Consequently, trace amounts of formaldehyde could be determined by high performance liquid chromatography even in co-existence of each aliphatic aldehyde.
