Determination of copper(II) at sub-picogram level by flow injection method using micellar enhanced chemiluminescence of 1, 10-phenanthroline

Application to rabbit lens

Abstract

The micellar enhanced chemiluminescence of 1, 10-phenanthroline-flow injection method was applied to determine trace amounts of copper(II) in rabbit lens; the distribution of copper(II) in the lens was first reported. The lens was dissolved with conc. HCl by heating, and the sample volume was adjusted to 2.5 ml by adding distilled and deionized water. The copper (II) contents in the lenses were measured through the standard addition method and the calibration curve method. The values agreed well between both methods; the average content was 3.3 μg per 1 g of the lens. It was first confirmed by the proposed method that copper(II) distributed homogeneously in the lens. The measurement can be performed with only 4μg of the lens in 2.5 ml sample solution. The limits of determination and detection for copper(II) in the lens, were 0.11 and 0.012 pg (20μl injection), respectively. The linear dynamic range for the copper(II) was about 104. The selectivity was very high; lead(II) and zinc (II), the strongest enhancers after copper(II), provided signals 0.01% of that for copper(II). Analysis time required was very short; less than 10s. The relative standard deviation was less than 1.9% for 10 repeated tests by 6.5×10^-10 M copper(II) injection. For the cross-check between atomic absorption spectrophotometry and the present method, the correlation coefficient 0.94 (n=8) was obtained.