1986 Volume 35 Issue 4 Pages T29-T32
A simple method was developed for the determination of total rnucopolysaccharides (MPS) commonly found in human urine. It is based on the separation of urinary MPS with cellulose column and the determination of the carboxyl group of MPS by colorimetry. MPS were separated from human urine as follows : To 20 ml of human urine, 4 ml of aqueous 0.1% (w/v) cetylpyridiniumchloride solution was added, and the mixture was charged onto a cellulose column (8 mm I.D. × 35 mm). After washing with 20 ml of distilled water and 30 ml of ethanol saturated with sodium chloride, urinary MPS were eluated with 9 ml of distilled water. MPS were determined by colorimetry based on the reaction of carboxyl group with o-nitro phenylhydrazine (ONPH) in the presence of 1-ethyl3- (3-dimethylaminopropyl)carbodiimide. Recovery of chondroitin-4-sulfate added to human urine by the present pretreatment was 92.5%. The determination method of MPS using a reaction with ONPH was correlated to the carbazole method.
